![]() ![]() Look for more examples of fibroblasts and note that you rarely see much cytoplasm and that the nuclei of these cells can be quite condensed and hyperchromatic depending upon the plane of section or the metabolic state. Note that the nucleoplasm of a fibroblast has a generally fine stippled (dot-like) chromatin pattern with occasional coarse chromatin clumps (heterochomatin) and one or two nucleoli. These are fibroblasts View Image (as opposed to clusters of similar appearing elongate nuclei that are usually smooth muscle cells or Schwann cells in a nerve that you will learn to recognize soon). Look for elongated nuclei, usually solitary, from which a modest amount of tapered cytoplasm extends from either one or both poles of the nucleus. The background will be clear, reflecting a "ground substance"-rich connective tissue. The collagen of fibrous supporting tissues, the dermis of the skin, tendon, ligaments and bone is type I collagen, which provides tensile strength. In this area note the irregular, wavy collagen fibers arranged singly or in small groups. Look at the connective tissue in the submucosa which is the lighter staining area View Image between the intestinal epithelium and the smooth muscle layer. Slide 29 (small intestine, H&E) View Virtual Slide Please read the descriptions of the connective tissue stains BEFORE you do your lab work.Be able to recognize a basement membrane (or basal lamina) in sections or micrographs where the structure is conspicuously present and understand its functions.Be able to recognize different types of connective tissue ( e.g., dense irregular, dense regular, loose, adipose) and provide examples where they are found in the body.Be able to use knowledge about the physical characteristics of collagen and elastin in explaining the functions of tissue where these molecules occur in large quantities ( e.g., coarse type I collagen fibrils present in dense connective tissue compared to more delicate type III fibers found closer to the interface of cells and the extracellular matrix).Be able to distinguish between type I collagen, type III (reticular) collagen, and elastic fibers when appropriately stained material is presented. ![]() Be able to recognize interstitial (fibrillar) collagens and elastic fibers at the light and electron microscopic levels.Be able to describe the functions of cells commonly found in connective tissue and identify them. ![]()
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